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Invitrogen™ Vitamin D3 Competitive ELISA Kit

Product Code. 30825456
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96 Tests
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30825456 96 Tests
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Product Code. 30825456 Supplier Invitrogen™ Supplier No. EEL151

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ELISA

Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is synthesized in the skin upon exposure to ultraviolet B (UVB) light from the sun. It is the most biologically active form of vitamin D and plays a crucial role in maintaining overall health. Structurally, vitamin D3 is a steroid hormone precursor that undergoes metabolic processes in the liver and kidneys to be converted into its active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body. The main function of vitamin D3 is to facilitate the absorption of calcium and phosphorus from the intestine, promoting their utilization for bone health and growth. It aids in the regulation of bone mineralization, ensuring the proper formation and maintenance of strong and healthy bones. Vitamin D3 is also involved in various other physiological processes beyond bone health. It plays a vital role in modulating immune function, helping to regulate the immune response and reduce the risk of autoimmune diseases. Additionally, it has been linked to cardiovascular health, muscle function, and mood regulation. In terms of localization, vitamin D receptors are present in various tissues throughout the body, including the intestines, bones, kidneys, immune cells, and brain. This widespread distribution of vitamin D receptors highlights the importance of maintaining adequate vitamin D3 levels for optimal functioning of these tissues and overall health.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 1.56 to 100 ng/mL
Assay Sensitivity 0.94 ng/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Vitamin D3
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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