Bioluminescence Assays and Reagents
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Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)
Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.
Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
Simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
| Substrate | Coelenterazine, D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Thermo Scientific™ Pierce™ Firefly Signal Enhancer (100X)
Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.
Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit
Perform firefly luciferase assays in high-throughput screening applications.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Detection Method | Bioluminescence |
Thermo Scientific™ pTK-Green Renilla Luc Vector for Luciferase Assays
Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
Thermo Scientific™ TurboFect™ Transfection Reagent
Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.
| Type | Transfection Reagent |
|---|
Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters
| Compatible Cells | Mammalian Cells |
|---|---|
| Substrate Type | Luciferase Substrate |
| Detection Method | Bioluminescence |
| Promoter | EF-1α |
| Substrate | Coelenterazine |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ pMCS-Red Firefly Luc Vector for Luciferase Assays
Drive red-shifted firefly luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
Thermo Scientific™ pTK-Gaussia-Dura Luc Vector for Luciferase Assays
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Thermo Scientific™ pTK-Gaussia Luc Vector for Luciferase Assays
Drive Gaussia luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).