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Description
The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.
Highlights
- Sensitive—highly sensitive detection of Cypridina luciferase activity
- Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
- Stable—reagents provide increased signal stability compared to flash assays
- Simple—does not require a luminometer with injectors
- Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
- Automation-friendly—amenable to high throughput assays
- Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
- Safe—allows one to perform non-radioactive assays
Requires
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers
Recommended applications
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Specifications
Specifications
| Type | Assay Kit |
| Target | Luciferase, Cypridina Luciferase |
| Quantity | 100 Reaction Kit |
| Content And Storage | • Cypridina Glow Assay Buffer, 5 mL, store at 4°C • Vargulin (1X), 5 μL, store at -20°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature Upon receipt store kit at -20°C or store individual components as indicated above. |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Format | 384-well plate, 96-well plate |
| Label Type | Enzyme Labeled |
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Safety and Handling
- Pierce Cypridina Luciferase Glow Assay Kit
- Danger
- Acute toxicity Category 3
- Serious eye damage/eye irritation Category 1
- Flammable liquid Category 2
- Skin corrosion/irritation Category 1 A
- Specific target organ toxicity after single exposure Category 1
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P243-Take precautionary measures against static discharge.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P363-Wash contaminated clothing before reuse.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : methanol
For Research Use Only. Not for use in diagnostic procedures.
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