Antibiotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN .05% EDTA

Gibco™ Penicillin-Streptomycin (5,000 U/mL)

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. PENICILLIN STREPTOMYCIN SOL

Gibco™ Cryopreserved Hepatocyte Recovery Medium (CHRM)

Cryopreserved Hepatocyte Recovery Medium (CHRM) 50 ML CRYOPRESERVED HEPATOCYTE RECOVERY MEDIUM(CHRM) 50mL store at 4°C

Gibco™ Penicillin-Streptomycin (10,000 U/mL)

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria PENICILLIN STREPTOMYCIN SOL

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media HEPES (1M) 100ML

Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas TRYPSIN 0.25% EDTA

Kanamycin Sulfate (White Powder), Fisher BioReagents

5GR Kanamycin Sulfate (White Powder)

Gibco™ Hygromycin B (50mg/mL)

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus 20 ML HYGROMYCIN B 20ML STORE AT 2-8C

Gibco™ B-27™ Supplement (50X), minus vitamin A

B-27 Supplement (50X), minus vitamin A 100ML B-27 SUPPLEMENT W/O VIT A (50X

Gibco™ 2-Mercaptoethanol (55mM)

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals 2 MERCAPTOETHANOL 1000X

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability 100 ML MEM NON-ESSENTIAL AMINO ACIDS SOLUTION10mM (100X), liquid 100mL RRR - pack at ambient

Gibco™ Insulin, human recombinant, zinc solution

Insulin, human recombinant, zinc solution INSULIN, RECOMBINANT HUMAN ZN

Gibco™ Zeocin™ Zeocin Selection Reagent

Selection antibiotic, formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus ZEOCIN 5G/50ML

Gibco™ N-2 Supplement (100X)

N-2 Supplement (100X) 50ML N2 SUPPLEMENT

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,

Gibco™ Fungizone™ Fungizone Antimycotic

Formulation of amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension 20ML AMPHOTERICIN B 250 UG/ML

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells INSULIN-TRANS-SEL-G, 100X

Ampicillin Trihydrate (Off-white Powder), Fisher BioReagents

25GR Ampicillin Trihydrate (Off-white Powder)

Gibco™ Primary Hepatocyte Maintenance Supplements

Primary Hepatocyte Maintenance Supplements CELL MAINTENANCE PACKCELL MAINTENANCE SUPPLEMENT B

Water, HPLC for Gradient Analysis, Fisher Chemical

HPLC for Gradient Analysis 1LT Water, HPLC for gradient analysis

Corning™ G418 Sulfate, Powder

Packaged in plastic bottles 5GR G418 Sulphate, Powder

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components 100 ML TRYPLE(TM EXPRESS IN PLASTIC CONTAINER,E-Z Hold(tm) RRR - pack at ambient store at 4°C

Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement 20ML L GLUTAMINE, 100X

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase Cell Dissociation Reagent STEMPRO ACCUTASEStemPro® Accutase® Cell Dissociation Reagent is

BD Bacto™ Difco™ Dehydrated Culture Media Additive: Yeast Extract, UF

Water-soluble extract of autolyzed yeast cells 500GR YEAST EXTRACT, UF

Gibco™ Trypsin (2.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN, 2.5%, 10X

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate), Powder

Produced by bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells GENETICIN

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures PLURONIC

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