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  8. 3,3',5,5'-Tetramethylbenzidine aq. soln., Thermo Scientific™

3,3',5,5'-Tetramethylbenzidine aq. soln., Thermo Scientific™

Product Code. 15493509
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Product Code. 15493509 Supplier Thermo Scientific Alfa Aesar Supplier No. J60808.AU
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Beschreibung

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Applications
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays. 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood assay of hemoglobin assay of peroxidases.

Solubility
Slightly soluble. <0.1 g/100 mL at 20°C.

Notes
Contains TMB in an aqueous buffer, pH 3.6 ± 0.2, with 0.03% hydrogen peroxide. The TMB product is soluble after conversion
TRUSTED_SUSTAINABILITY

Chemische Identifikatoren

CAS 54827-17-7
Molecular Formula C16H20N2
Molecular Weight (g/mol) 240.35
MDL Number MFCD00007748
InChI Key UAIUNKRWKOVEES-UHFFFAOYSA-N
Synonym 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
PubChem CID 41206
IUPAC Name 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
SMILES CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Spezifikation

Color Yellow
Quantity 1000 mL
Beilstein 2808541
Formula Weight 240.35
Physical Form Liquid
Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine
Name des Produkts
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