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CD45 Mouse, BUV395, Clone: D058-1283, BD

Product Code. 15833359
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50 Tests
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Product Code. 15833359 Supplier BD Biosciences Supplier No. 564099

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Description

Mouse Monoclonal Antibody

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45
Applications Flow Cytometry
Classification Monoclonal
Clone D058-1283
Conjugate Brilliant Ultraviolet 395
Description Pan Leukocyte, NHP-specific
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Rhesus peripheral whole blood
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Baboon, Cynomolgus, Rhesus Monkey
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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