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  7. Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG 

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Artikelnummer. 10311135 Shop alle Applied Biosystems produkter
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No. of Reactions:
1250 Reactions
250 Reactions
2500 Reactions
500 Reactions
5000 Reactions
Pakningsstørrelse:
1 set
1250 reactions
50mL
Each
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Artikelnummer. No. of Reactions unitSize
10311135 500 Reactions 1 set
10637755 5000 Reactions 50mL
10116674 250 Reactions Each
10575385 1250 Reactions 1250 reactions
10702697 2500 Reactions 1 set
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Artikelnummer. 10311135 Leverandør Applied Biosystems™ Leverandørnr. 4366072

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405.00 EUR valid until 2026-06-30
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Beskrivelse

Kræver

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

  • Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
  • The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
  • A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
  • Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
  • TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
  • Compatible with TaqMan Gene Expression assays
  • Store at 2°C to 8°C

Ordreinfo

Guaranteed minimum shelf life is 60 days.

TRUSTED_SUSTAINABILITY

Tekniske data

Concentration 2X
Content And Storage TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 μL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line AmpErase, TaqMan
Product Type Fast Universal PCR Master Mix
Quantity 500 reactions
Sufficient For 500 Reactions
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq) 2 X
No. of Reactions 500 Reactions
Sample Type DNA (Genomic), cDNA
Volume 20 μL
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Frequently Asked Questions (FAQs)

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

For Research Use Only. Not for use in diagnostic procedures.

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