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  7. Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG 

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

Product Code. 10420355 Shop All Applied Biosystems Products
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Product Code. 10420355 Supplier Applied Biosystems™ Supplier No. 4440045

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Description

Requires

Primers, probes, template, water

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG).

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Order Info

Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan
Product Type Universal Master Mix II
Purity or Quality Grade UP (Ultra Pure)
Quantity 5 x 5 mL
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq) 2 X
Sample Type DNA (Genomic), cDNA
Volume 55 mL
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Frequently Asked Questions (FAQs)

Do I have to use the Universal Master Mix with my TaqMan MicroRNA Assays? If so, which one?

Yes. This is the recommended master mix for TaqMan MicroRNA Assays. You can use Universal Master Mix or Universal Master Mix II (with or without UNG). You can also use the Fast Advanced Master Mix.
Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.
Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

For Research Use Only. Not for use in diagnostic procedures.

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