CD124 Mouse anti-Human, Brilliant Violet 510, Clone: hIL4R-M57, BD Optibuild™

Mouse Monoclonal Antibody

The hIL4R-M57 antibody specifically binds to the α subunit (IL-4R α) of the human Interleukin-4 Receptor complex (CD124). The human IL-4Rα, also known as B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines. The cell surface IL-4R α chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4R α/γ c; aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4Rα /IL-13Rα 1; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13. A truncated form of the IL-4Rα exists in soluble form in biological fluids. In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor. The serum levels of soluble IL-4Rα appear to elevate in pathological situations such as allergy and parasitic infections. Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4Rα may augment or antagonize the activities of IL-4. The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R.