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Invitrogen™ FITC Polyclonal Antibody
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Product Code. 10662214
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Quantity:
100 μg
Unit Size:
100µg
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10662214 100 μg 100µg
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Product Code. 10662214 Supplier Invitrogen™ Supplier No. 711900

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348.61 EUR valid until 2026-07-03
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Description

Rabbit Polyclonal Antibody

This antibody was purified from serum of rabbit hyper-immunized with FITC conjugated to KLH. The antibody was purified by antigen-specific matrix. The antibody reacts with FITC but not the carrier protein. Useful as a secondary antibody in a wide range of immunoassays including: ELISA, tissue and cell staining, immunoprecipitation, and western blotting. The protein concentration is determined based on an E (1%) = 14 at 280 nm.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
TRUSTED_SUSTAINABILITY

Specifications

Antigen FITC
Applications ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Classification Polyclonal
Concentration 0.25 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% sodium azide; pH 7.4
Gene Alias Fluorescein
Host Species Rabbit
Immunogen FITC.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Chemical
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