LAP (Latency Associated peptide) Monoclonal Antibody (VB3A9), Functional Grade, eBioscience™, Invitrogen™

Description

This VB3A9 monoclonal antibody recognizes human latency-associated peptide (LAP), also known as the TGF-beta propeptide. Human LAP is a homodimer of 65-75 kDa that is non-covalently associated with TGF-beta and this complex is also referred to as the small latent complex (SLC). LAP can be covalently associated with latent TGF-beta binding proteins (LTBP) through disulfide bonds, forming the large latent complex (LLC). The association of LAP with TGF-beta facilitates its secretion and also renders TGF-beta inactive. VB3A9 has been reported to cross-react with Non-human Primate LAP, but does not cross-react with mouse LAP. Applications Reported: This VB3A9 antibody has been reported for use in immunoblotting (WB) and immunoprecipitation. Applications Tested: This VB3A9 antibody has been tested by immunoblot analysis of human platelets under reducing conditions at 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

LAP (Latency Associated peptide) Monoclonal antibody specifically detects LAP (Latency Associated peptide) in Human samples. It is validated for Immunoprecipitation, Western Blot