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Invitrogen™ MHC Class I (H-2Kk) Monoclonal Antibody (AF3-12.1.3), Biotin, eBioscience™
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Product Code. 15229939
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Quantity:
100 μg
Unit Size:
100µg
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15229939 100 μg 100µg
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Product Code. 15229939 Supplier Invitrogen™ Supplier No. 13594082

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Description

Mouse Monoclonal Antibody

Description: This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF3-12.1.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF3-12.1.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
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Specifications

Antigen MHC Class I (H-2Kk)
Applications Flow Cytometry
Classification Monoclonal
Clone AF3-12.1.3
Concentration 0.5 mg/mL
Conjugate Biotin
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene H2-K1
Gene Accession No. P01901, P03991, P04223, P14428
Gene Alias class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H2DD; H2K; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; H2KK; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen
Gene Symbols H2-K1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 14972
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1
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