missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. 10342463

Invitrogen™ ZO-1 Polyclonal Antibody
GREENER_CHOICE

Product Code. 10342463
Change view
Click to view available options
Quantity:
100 μg
Unit Size:
100µg
Product Code. Quantity unitSize
10342463 100 μg 100µg
1 options
This item is not returnable. View return policy

Product Code. 10342463

Brand: Invitrogen™ 617300

Please to purchase this item. Need a web account? Register with us today!

Edge
551.00 EUR valid until 2026-02-28
BEST PRICE on Promo! Use promo code "28033" to get your promotional price.
This item is not returnable. View return policy

Description

Rabbit Polyclonal Antibody

61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA and Immunohistochemistry. To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required. The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes. Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody. Frozen sections have been successfully stained after fixation with acetone at 4°C.

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
TRUSTED_SUSTAINABILITY

Specifications

Antigen ZO-1
Applications ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 0.25 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% sodium azide; pH 7.4
Gene TJP1
Gene Accession No. O97758, P39447, Q07157
Gene Alias DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
Gene Symbols TJP1
Host Species Rabbit
Immunogen A 69 kD fusion protein(1) corresponding to amino acids 463-1109 of human ZO-1 cDNA.(2) This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100718142, 21872, 292994, 403752, 7082
Target Species Canine, Guinea Pig, Human, Mouse, Monkey, Rat
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.